ASSOCIATE RESEARCH SCIENTIST COVER LETTER TEMPLATE

• Work with the Early and Lead biology staff to express
• Purify novel gene-editing proteins for the discovery pipeline.
• Perform medium and small-scale bacterial expression 
• Affinity purification of novel bacterial enzymes for downstream development.
• Contribute to the inventory of samples for internal and external projects.
• Perform and refine purification protocols for new and lead enzymes
• Generate high quality protein for lead validation and preclinical studies.
• Analyze protein products for purity and contaminants, ie endotoxin and nucleic acids.
• Prepare accurate and timely data summaries, contribute to the writing of reports 
• Present findings to key stakeholders
• Lead by example in the area of safety behaviors by promoting safe work habits.

Skills: Protein Expression, Expression Specialist, Purification Expert, Inventory Manager, Protocol Specialist, Protein Producer, Analysis Technician, Safety Advocate

• Synthesis, purification, and characterization of proprietary DNA Encoded Libraries
• Application of synthetic chemistry knowledge to evaluate reactions and reagents for on-DNA, aqueous synthesis
• Apply plate-based parallel synthesis strategies in a split-and-pool manner
• Apply automated synthesis skills using Tecan liquid handler to make DELs.
• Analyze, interpret, and report experimental results at chemistry and program meetings.
• Maintain detailed records of experiments in electronic lab
• Contribute to conceptions, presentations, publications, and patents
• Perform a wide variety of basic molecular biology techniques with minimal supervision.
• Analyze pertinent data sets and communicate results to the team
• Attend Proteomics team meetings and provide regular updates to team members
• Document the experimental plan, lab work and any deviation, analyze the data and present the results for discussion

Skills: DNA Synthesis, Chemistry Analysis, Synthesis Strategy, Synthesis Automation, Data Analysis, Lab Recording, Biology Technician, Team Contribution

• Culturing primary and immortalized cell lines and performing in vitro and ex vivo experiments
• Protein characterization techniques such as ELISAs and Western blot analysis on biofluid and tissue lysate samples
• Paraffin embedding and histological analysis of key tissues, prepare reagents kits
• Preparation of tissue samples and use of qRT-PCR to measure mRNA species
• Analyzing data, preparation of Powerpoint presentations
• Presentation of research findings in group and departmental meetings.
• Time-appropriate recording of data in a laboratory notebook
• Establish reagent development and manufacturing protocols and forms under general guidance
• Develop reagent QC test methods and performance specifications
• Perform reagent stability and guard-banding studies according to written protocols and study plan
• Collaborate and communicate effectively in a highly diverse cross-disciplinary environment

Skills: Cell Culture, Protein Analysis, Histology, Molecular Biology, Data Analysis, Presentation, Notebook Management, Reagent Development, Collaboration.

• Lead and perform NGS operation and maintenance for sequencers and robotic systems
• Play a lead role with lab organization, freezer/refrigerator cleanup and organization, purchasing and organization of supplies, etc.
• Work independently and/or in collaboration with other team members on new assay and protocol development.
• Independently perform diverse methods for the extraction, purification and quantification of DNA and RNA from cells and archival tissues.
• Prepare and able to troubleshoot NGS library constructions for bulk DNA and RNA samples and single cell analyses.
• Maintain an accurate and complete laboratory notebook and other records 
• Support tracking of projects with external vendors 
• Contribute to ongoing scientific discussions and prepare regular updates on projects.
• Enter data into company databases and data storage files.
• Develop and edit protocols and standard operating procedures.

Skills: NGS Operations, Lab Organization, Assay Development, Nucleic Acid Handling, Library Construction, Record Keeping, Vendor Coordination, Scientific Communication

• Develop and establish automation-specific SOPs for relevant proteomics sample prep protocols. 
• Execute established high throughput protocols.
• Program and generate plate definitions (measurements) for use on multiple automation platforms currently within the proteomics group
• Establish liquid class information for various proteomics workflows
• Optimize liquid classes for use on proteomics lab automation platforms
• Operate and monitor automation equipment and optimize said workflows
• Diligently tracking as well as resolving issues that may arise
• Generate and program work lists for automation platforms from defined proteomics experimental designs
• Collaborate and partner with Proteomics group members to establish specific use cases and associated automation needs
• Analyze automation centric datasets and associated metrics using computational tools such R and python
• Develop and establish criteria for formatting and selecting relevant data and meta data for ingestion into Proteomics specific LIMS
• Maintain electronic laboratory notebooks

Skills: SOP Development, Protocol Execution, Automation Programming, Liquid Handling Optimization, Workflow Optimization, Equipment Operation, Issue Tracking, Worklist Generation

• Extensive human cell culture experience, experience with flow cytometry
• Experience with multiplexed ELISA instruments like Luminex
• Solid knowledge of modern molecular and cell biology lab techniques
• Hands-on experience with fluorescence microscopy
• Excellent English-language communication skills
• Ability to work in the United States without sponsorship
• Time management and project management skills
• Good written and oral communication skills
• Ability to work in a collaborative work environment with a team, ability to train junior staff
• Proficiency in assay development and qualification desired.

Qualifications: BS in Biology with 2 years of Experience

• Experience with flow cytometry, knowledge of cGMP guidelines 
• Experience with mammalian cell culture and aseptic technique is highly desirable.
• Experience with BD FACSDiva and/or Miltenyi MACSQuantify flow cytometry software
• Experience with gene therapy vectors and/or gene mediated cell therapy is desirable.
• Good ability to apply feedback received for process improvement
• Required to give formal technical progress/project reports to RandD
• Manages own time and is capable of managing technician work time/workflow
• Deliver high quality data in a timely and well-organized manner.
• Ability to handle multiple projects and work in a fast-paced dynamic team environment
• Flexibility to adapt to changes with a positive attitude.
• Able to write protocol, conduct test, analyze result and write report.

Qualifications: BS in Chemistry with 4 years of Experience

• Previous experience that provides the knowledge, skills, and abilities to perform the job
• Knowledge of general chemistry, separation science (HPLC, UPLC, gel and capillary electrophoresis)
• Experience in leading CMC studies for biopharmaceutical products
• Ability to independently perform root cause analysis for method investigations
• Proficiency in technical operating systems, proven ability in technical writing skills
• Proven problem solving and troubleshooting abilities
• Time management and project management skills, good written and oral communication skills
• Ability to work in a collaborative work environment with a team, ability to train junior staff
• Adherence to performance review and goal setting process
• Proactively seeks guidance from other team members 

Qualifications: BS in Biochemistry with 5 years of Experience

• Research experience in cancer biology and cancer immunotherapy is highly desirable.
• Hands-on experience in isolation, culture, and characterization of primary cells from human or animal tissues 
• Experience executing biochemical assays with a variety of readout technologies, including flow cytometry and ELISA 
• Excellent communication, presentation, collaboration and organizational skills 
• Ability to execute and operate to a high standard.
• Attention to detail with excellent organizational and record keeping skills
• Ability to identify technical problems and potential solutions.
• Proficiency in utilizing software such as Graph Pad, Microsoft Office, etc. for data analysis and figure generation 
• Normally receives general direction on all projects and assignments
• Demonstrates ownership of, and accountability for, assigned project tasks

Qualifications: BS in Molecular Biology with 3 years of Experience

• Experience in small molecule drug discovery process in an industrial setting
• Understand key aspects of SAR support using biological assays.
• Familiar with and willing to learn in vitro cell-based assays, including but not limited to: cell proliferation assays, cell cycle assay, apoptosis assay, bDNA assay, image-based assays.
• Sophisticated experience in cell biology including mammalian cell culture and characterization, lentivirus production, transient/stable transfection
• Familiar with/willing to learn gene editing including CRISPR - KO and - KI.
• Experience in biochemistry (Protein assays, Western blot, ELISA), and molecular biology (DNA cloning, PCR, qPCR).
• Familiar with various biology laboratory equipment with an emphasis on automation 
• Capable of troubleshooting for SAR assay support.
• Excellent communication, collaboration, and organizational skills.
• Capability to stay focused, multitask, attention to details 

Qualifications: BS in Biotechnology with 5 years of Experience